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l**********1
发帖数: 5204 | 2 奇了 怪了 没啥人曝这条吗 快一周了 哈
09/05/2012
Glasgow’s Beatson Institute investigating circumstances of Cell retraction
for inappropriate images
with 6 comments
The Beatson Institute for Cancer Research in Glasgow, Scotland is looking
into how inappropriate images ended up in a Cell paper that has just been
retracted.
Here’s the notice for the paper by Lynne Marshall, Niall S. Kenneth, and
Robert J. White:
This article has been retracted: please see Elsevier Policy on Article
Withdrawal http://www.elsevi... 阅读全帖 |
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m******5
发帖数: 1383 | 3 Dear All:
I got a mice by hand with specific genetic trait, I want to get a homozygote
mES cell line from it. Knowing that we could not isolate mES cell from
blastocyst in our facility, I tunred to iPS or nuclear transfer.
IS there known facility out there provide service that we could make iPS or
nuclear transfered ES cell in a reasonable price range? thanks!!! |
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w*******e
发帖数: 30 | 4 最近闲来无事整理了几个journal近二十年来的影响因子的统计（如图）
发现PNAS比较稳定，一直是10左右的水平，
nature/science 确实不相上下，缓慢稳步增长
不过cell和new england journal of medicine却在2000年左右有较大变化
2000年以前cell都是比NEJM高的
但是2000年以后NEJM越来越牛，cell却越来越不行了
2000年实现反超，从此差距越来越大
这背后有什么故事么？是因为cell有子刊的影响么？还是说NEJM有什么特别牛的策略？ |
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i*******u
发帖数: 5 | 5 Link: http://www.cell.com/spotlightonchina
The 2013 Spotlight on China is an informational supplement to the journal
Immunity that highlights recent developments in immunology that are the
culmination of investments by the Chinese government, Chinese divisions of
pharmaceutical companies, and Chinese scientists. The essays showcase
advances in the understanding of both innate and adaptive immunity as well
as efforts to translate these findings into tackling autoimmune disorders
and infectious d... 阅读全帖 |
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s******a
发帖数: 252 | 6 The golden age of metabolism is coming again.
The signaling roles of metabolites have been unappreciated for too long,
even prominent examples like cAMP are everywhere.
Stability and low concentration limit the study of many of them. We may know
less than half of metabolites in our body right now.
High resolution mass spectrometers and systems biology will power the next
wave of discoveries. The fist half of 20th century was the first golden age.
Pasted below are Nobel prizes related to metaboli... 阅读全帖 |
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s******a
发帖数: 252 | 7 The golden age of metabolism is coming again.
The signaling roles of metabolites have been unappreciated for too long,
even prominent examples like cAMP are everywhere.
Stability and low concentration limit the study of many of them. We may know
less than half of metabolites in our body right now.
High resolution mass spectrometers and systems biology will power the next
wave of discoveries. The fist half of 20th century was the first golden age.
Pasted below are Nobel prizes related to metaboli... 阅读全帖 |
|
j********r
发帖数: 156 | 8 碰巧刚看到几篇关于氨基酸代谢的文章，大牛来点评一下。
1. Serine starvation induces stress and p53-dependent metabolic remodelling
in cancer cells （nature, 2013)
2. Serine, but Not Glycine, Supports One-Carbon Metabolism and Proliferation
of Cancer Cells (Cell reports, 2014)
3. Phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to
oncogenesis (Nature genetics, 2011)
4. Functional genomics reveal that the serine synthesis pathway is essential
in breast cancer (nature, 2011)
elife刚发了一片关于metformin的文章。俺愣是没看... 阅读全帖 |
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a*****n
发帖数: 2835 | 9 请教版上有没有朋友做过细胞直径分布测量实验的，cell size distribution.
研究TSC-mTOR signaling 的经常要测量细胞直径，有人用流式细胞仪，也有不少用
cell counter的。
我最近做了几次，用的是cell counter，得到的细胞直径分布图一般都是不规则的曲线
，而文章里面的一般是类似正态分布曲线图
请问有这方面经验的版友，这个实验有什么关键的注意事项?
附图1是截取一篇cell文章里的结果
附图2是我自己的一个结果
多谢！ |
|
f*****f
发帖数: 195 | 10 就实验研究手段而言，两者有啥区别？
satellite cell可以认为是muscle stem cell，我看到paper上大部分都是小鼠in vivo
的实验。primary myoblast实际上也是将satellite cell传代分离得到的。那么，假如
体外培养muscle stem cell，不就变成了myoblast?
谢谢！ |
|
z****u
发帖数: 1007 | 11 不是很明白你这个问题。
传统说法是Pax7+ satellite cell变成myogenic cells, proliferate.. primary
myogenesis
then secondary myogenesis, myoblast cluster and form the myotubes.
体外养satellite cells的方式好像很局限。我还没看到好的protocl能体外扩增
satellite cell without losing the undifferentiate status.
vivo |
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l**********1
发帖数: 5204 | 12 she is as 1000 tallent now in tg/wan wan already ? !!
from
HTTP : //www.sciencedirect.com/science/article/pii/S1535610803000308
and
Cancer Cell Elsevier
www.elsevier.com/journals/cancer-cell/1535-6108Cancer Cell publishes reports
of novel results in any area of cancer research, from molecular and
cellular biology to clinical ...
5 year impact factor: 28.17
HTTP : //www.google.co.uk/search?hl=en&tbo=d&output=search&sclient=psy-ab&q=
cancer+cell+impact+factor+&btnK= |
|
d********r
发帖数: 211 | 13 1) 这个转基因的T cells变成memory T cell后有多久的记忆能力? 可能以后记忆消失
癌症又复发吗?
2) 转基因的T cells有可能的副作用吗? 会不会变成新的tumor T cell?
ip |
|
d*p
发帖数: 534 | 14 t 细胞就是在血液，淋巴，组织只见循环， 血液和淋巴是最富集的地方，细胞回输通
过静脉，直接进入血液，也就直接接触血液癌细胞，因此不存在migration,
infiltration问题。
固体肿瘤会形成一个屏障，阻止t cell 的进入，同时固体肿瘤内部是一个非常强的免
疫抑制机制，进去的t cell 也很容易被抑制，也就是说，有限t cell 能够进入，进入
后功能又被抑制。因此在固体肿瘤上的效果不好。通过提高t cell 进入和抵抗肿瘤抑
制环境能力，就可以提高效果。
抗原当然也是一个原因，相比前面说的两个原因， 重要性底点，因为现在已经鉴定了
不少肿瘤抗原了。
cd19 car 是把几乎所有b 细胞都干掉，固体肿瘤里这样的抗原比较少，有也通常是组
织特异性的，会累计整个组织，器官。 |
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l**********1
发帖数: 5204 | 15 十年后 那学校有关生物医学的会说 The Lancet, NEJM 只是临床医学领域的
Cell是生物医学多领域杂志
把Lan nejm 抬到Cell 等同的地步，有点降低Cell的档次..lol
cell |
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s*******e
发帖数: 1389 | 16 我是纯外行，做果蝇发育的。你这两篇加上更早的这两篇：
（Submitted 16 February 2012; accepted in final form 7 May 2012）
Ren G, Kim JY, Smas CM (2012) Identification of RIFL, a novel adipocyte-
enriched insulin target gene with a role in lipid metabolism. Am J Physiol
Endocrinol Metab 303(3):E334–E351.
(Received 8 July 2012, available online 15 July 2012)
Ren Zhang （2012）Lipasin, a novel nutritionally-regulated liver-enriched
factor that regulates serum triglyceride levels。 BBRC
草草看完后，
1，先说命名。
"This gene (RIFL) was variously... 阅读全帖 |
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s*******e
发帖数: 1389 | 17 我是纯外行，做果蝇发育的。你这两篇加上更早的这两篇：
（Submitted 16 February 2012; accepted in final form 7 May 2012）
Ren G, Kim JY, Smas CM (2012) Identification of RIFL, a novel adipocyte-
enriched insulin target gene with a role in lipid metabolism. Am J Physiol
Endocrinol Metab 303(3):E334–E351.
(Received 8 July 2012, available online 15 July 2012)
Ren Zhang （2012）Lipasin, a novel nutritionally-regulated liver-enriched
factor that regulates serum triglyceride levels。 BBRC
草草看完后，
1，先说命名。
"This gene (RIFL) was variously... 阅读全帖 |
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m****a
发帖数: 427 | 18 比如 osteogenic cell line MC3T3-E1和 immortalized primary osteoblasts(自己
isolate and immortalize 的 osteoblasts)
要做一个实验，要拿cell line还有immortalized primary cell做对比，但我自己感觉
都是已经被immortalized的，就没什么区别了。值得试吗？
这两种细胞会有区别吗？谢谢啦 |
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q******g
发帖数: 3858 | 19 Molecular cell不见得比Nature cell biology差吧。个人的喜好不同。感觉一个档次
的杂志。cell metabolism差些。 |
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a******u
发帖数: 211 | 20 Cell一直是想挣大钱的啊！Cell Reports不见得比Cell research IF高，至少再5-10年
之内应该不会发生。你随便看看Cell reports里面的文章的实时的citation就知道了，
相当不怎么样。这个和文章的质量无关。Nature好多文章都很bullshit, 但是不影响
citation. 这个是辩证关系和心理作用：同样的东西发在plos one上面就不如CNS上面
的citation高。大家都习惯cite high-profile journal papers. |
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l*******r
发帖数: 27 | 21 我是发过cell research的，也帮老板审过该杂志的文章。
我的感觉这个杂志在审稿上还是比较专业的，尽管不排除有些文章编辑格外青睐，但是
绝大部分审稿过程还是很正式的。
发文章给钱这事楼主就不要去道听途说了！我是听都没听过。
再说了，你会为了区区3万块钱人民币，把你的一篇Cell投到Cell research上？都不说
cell了，NCB都不会有人干的。
有些事情用钱是能搞定的，但不是所有的事情。
每个杂志都有他们自己的几把刷子，要不咋在江湖混？ |
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s******h
发帖数: 23 | 22 http://www.bbc.com/news/health-26516458
A Japanese scientist behind a seemingly groundbreaking stem cell study says
the findings should be withdrawn amid doubts over its quality.
It was reported in January that dipping cells in acid could cheaply and
quickly convert them into stem cells.
But questions were raised about the images used in the scientific report and
other research groups have failed to reproduce the results.
Author Prof Teruhiko Wakayama said: "It is no longer clear what is right."... 阅读全帖 |
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t**l
发帖数: 109 | 23 不过她们家的文章都很有意思，做神经行为方向，全是western blot 生化方向的。 很
少有组织学，生理学的实验。当然最后总会用一个行为学的实验来验证一下genetic
phenotypye,剩下的就是用生化解释pathway。感觉CELL老多都是这样的PAPER。起点都
是一个IN VIVO或者primary cell tissue, 中间做着做着，就看见293T cell, 然后
FLAG-TAG。我现在一看见有293T cell的文章，感觉都头大。那些生化PATHWAY用293T解
出来的，到底有多少是能真实的反应in vivo的生理。 |
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j********r
发帖数: 156 | 24 how about the following submission order
Nature cell bio
G&D
Mol Cell / Embo J
eLIFE
Current Bio / Dev Cell / Science Advances
Cell Rep/Embo Rep |
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j********r
发帖数: 156 | 25 how about the following submission order
Nature cell bio
G&D
Mol Cell / Embo J
eLIFE
Current Bio / Dev Cell / Science Advances
Cell Rep/Embo Rep |
|
l*******i
发帖数: 153 | 26 照着Cell Stem Cell与Stell Cell Report的Editorial Board找就行了。stem cell的
牛人基本上都在这两个Board里了。
另外，干细胞，特别死胚胎干细胞与iPSC领域内的问题，很大一部分不是由于人为造假
，而是由于干细胞的特性决定的。与其他的细胞不一样，干细胞对enviroment的适应性
太差，很容易就会发生改变，而且很多时候，仅凭少数的评价指标（比如morphology,
marker gene expression等）根本就区分不出来。
但不可否认的是，干细胞确实是一个非常好的modeling platform；最近几年，生物与
基础医学领域的许多大的突破，都得益于干细胞的研究，比如epigenetics, NGS的应用
，translation medicine的发展，等等。 |
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t****w
发帖数: 130 | 27 仅仅依靠一个两个所谓marker基因的相对表达水平来鉴定cell identity是阻碍干细胞
领域发展的最大障碍之一。
看上去很美好的Lineage tracing系统其实问题挺多，最大的问题就是干细胞往往非常
rare,很难detect到。而你detect到大量label成功的细胞不一定是真正发挥功能的干细
胞。
今后的希望还是回归到最本质的问题：what is cell? 细胞是执行生物功能的一个功能
单位，所以cell identity还是要从功能鉴定入手。所谓stem cell,不在于是否表达
marker，而在于在体内（或体外）是否有self-renewal的能力，是否能在分化过程中产
生出其他生物学功能。
关于功能检测方法多多，可操作性强一点的就是看看几个功能相关基因的表达。硬要讨
论“marker”的话，几个功能相关的基因协同表达倒可以算是个有意义的“marker set
”。 |
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f******g
发帖数: 1003 | 28 我最近发现一个membrane protein （A), FACS 抗体是能够买到的。
A 阳性的细胞只有total bone marrow的1%。in A positive cells, 30-50% is KSL
cells.
Transplantation assay shows that A positive cells are HSC enriched cells.
A ko mice are viable, but HSC function in A ko mice is not investigated yet.
I plan to generate a reporter mice, do you think will it be interesting? |
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j*********o
发帖数: 237 | 29 先多谢了！！
Tao Deng,Ph.D
Email: [email protected]
/* */
Research Fellow
National Cancer Institute
National Institutes of Health
Summary of Research Experience:
Deep understanding of epigenetic regulations, including DNA methylation,
histone modifications and chromatin dynamics
Rich experience on mouse embryonic stem cell manipulation
Full hands-on experience on Next-generation sequencing
Selected Publication:
1. Deng T*, Zhu ZI*, Zhang S, Postnikov Y, Huang D, Horrsch M, Furusawa T,
Beckers J,... 阅读全帖 |
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x*******t
发帖数: 3764 | 30 Cell report距离子刊都还差很远吧，能中neuron或者nature neuronscience的没人会
考虑cell report的。
cell
车。 |
|
发帖数: 1 | 31 Science, Nature, Cell 三刊齐名，同一档次。大家口中常叨咕的NCS的C，就是Cell。
Cell只发生命科学文章，Science，Nature还发别的学科的文章。不懂就问是好的。
2016年
Science IF = 34.66
Nature IF = 38.138
Cell IF = 28.710 |
|
发帖数: 1 | 32 impact factor是和学科相关的。
对二流的期刊，业内评价就可以，但是对一流的期刊，业内评价有用吗？要更大的同行
评价才行。
期刊最终要转为经费才能衡量，生物界自己评价cell水平高，人家不给钱没用啊。各个
学科一起去金主争经费的时候，生物界认可cell牛逼有什么用啊？还不是要外行认可才
行。cell每年再多发一倍的文章，只能让cell的文章贬值，而不能让生物界的经费增加
一倍啊 |
|
|
f*******e
发帖数: 354 | 34 t cell b cell 都做过了.
2010 Cell stem cell, 3 papers from rudolf jaenisch, george daley, and
another lab
: 不好意思，没找到相关文章，你能说的详细点吗？
|
|
发帖数: 1 | 35 Mol system bio圈外人知道的少。不如投nature communications啊，综合性的，影响
因子不会掉，知道这个期刊的人也多
cell systems不会超过genome research
：system biology的杂志还有mol system bio,也是十几分.
：还是祝贺楼主的工作，能投上10分的都不容易，cell press的刊物都挺折腾人的。 |
|
b**********e
发帖数: 108 | 36 A postdoctoral position in Microfluidics for Single-Cell Analysis
The project is focused on developing new microfluidic platforms and
molecular bioassays for quantitative single-cell analysis of cancer. Their
applications to cancer pathology and clinical diagnostics will be pursued in
collaboration with KU Medical Center. A recent Ph.D. in chemistry,
biochemistry, molecular/cell biology, bioengineering or biomedical sciences
is required. The applicants should have strong interest and expertise i... 阅读全帖 |
|
b**********e
发帖数: 108 | 37 A postdoctoral position in Microfluidics for Single-Cell Analysis
The project is focused on developing new microfluidic platforms and
molecular bioassays for quantitative single-cell analysis of cancer. Their
applications to cancer pathology and clinical diagnostics will be pursued in
collaboration with KU Medical Center. A recent Ph.D. in chemistry,
biochemistry, molecular/cell biology, bioengineering or biomedical sciences
is required. The applicants should have strong interest and expertise i... 阅读全帖 |
|
H*****l
发帖数: 702 | 38 包子？
我搓，才来MITBBS，不知道什么是包子。。。
i agree with you
it is very strange, not many solar cell companies have good R&D groups
my research realm is silicon heterojunction solar cell(not mid-bandgap part
in a tandem cell but a-si/c-si solar cell) and.... play with new device
structure and fast process
i knew United Solar/First Solar/Sunpower......
but there are very limited vacancies in their R&D groups.
And i noticed that many solar guys finally jumped back to traditional
semiconductor area after 1or 2 ye |
|
a***u
发帖数: 9 | 39 Currently, the highest power efficiency is around 33% (tested at NREL). It is
a tandem cell with three junctions. The highest efficiency was achieved at 300
suns (AM1.5). It is certainly true that the built-in potential of inorganic
p-n junction cell sets the maximum limit for Voc. But this is not true for
organic cell. Even for symmetrical organic cells sandwiched between two
identical electrodes, the Voc will not be zero. Brian Gregg wrote an excellent
review paper on this topic. (JPC B, 2003, |
|
z****n
发帖数: 589 | 40 【 以下文字转载自 THU 讨论区 】
发信人: youxi (youxi), 信区: THU
标 题: Fuel cell modeling Postdoc positions
发信站: BBS 未名空间站 (Thu Sep 11 13:23:47 2008)
Fuel cell modeling Postdoc positions
Two post-doc research fellow positions are available at the center of solid
oxide fuel
cells at the University of South Carolina http://www.engr.sc.edu/SOFC/team.html.
The research area is about solid oxide fuel cell modeling and computation or
closely related topics. At least one of the following primary Background is
preferr |
|
y********4
发帖数: 33 | 41 Hi All,
Who could kindly provide or lend a BlackBerry Cell Phone Charger. I bought
BlackBerry Cell Phone (7100i) from ebay,they did not provide a charger. If
you are or used to be a customer of BlackBerry, your cell phone charger will
be compatible with Blackberry 7100i Cell Phones. Please drop a email to
j********[email protected] if you like to do it.
Thanks!
Junzuo Wang |
|
z*******n
发帖数: 1034 | 42 Lee Mathews Nov. 14, 2014 12:28 pm
What do Iraqi insurgents and U.S. citizens have in common? Both have been
targeted by aerial spying programs that use fake cell towers. It’s been
going on since 2007.
A report from The Guardian details a so-called “dirtbox” program, employed
by the Justice Department and U.S. Marshals to track movements of suspected
criminals. Authorities shove the dirtbox — a device that pretends to be an
ordinary cell phone tower — into a Cessna, then fly around the country
l... 阅读全帖 |
|
l*******r
发帖数: 39279 | 43 【 以下文字转载自 Biology 讨论区 】
发信人: zxt (Asoka & Akaba), 信区: Biology
标 题: Re: 大家说说Cancer cell metabolism吧，这个越来越火啊！
发信站: BBS 未名空间站 (Sun Nov 4 01:53:57 2012, 美东)
在做相关项目，上来胡说几句，当是抛砖引玉，大家多多指正
otto warburg老早就发现cancer更喜欢glycolysis，还因此得了诺奖，之前的肿瘤代谢
主要在体外造影上有一定的应用，为啥肿瘤代谢要到现在又火了呢。在这个field里真
正让代谢又火起来的个人看来是三个topic: PKM2, IDH1/2, Lkb1/mTor/AMPK。
尤其是Lewis Canteley 2008 Pkm2的nautre，让人们知道了cancer cell是如何建立
Warbug
effect的，这之后各种可能和warburg effect相关的pathway又被人们陆续挖掘了出来
。而通过对PKM2/LDHB等glycolysis相关酶的knockdown, tumor cell的生长... 阅读全帖 |
|
w*******y
发帖数: 60932 | 44 All credit goes to Kralik @ FW
Sony is doing an electronics recycling program here:
Link:
http://recycling.econewonline.com/sonystyle/?q=main
They will give you a variety of trade-in ecoupons guaranteed, from $30 for
an old MP3 player to $100 for a laptop/netbook. The DEAL is that they will
send you the e-coupon before they ever receive your trade-in. I have no idea
how they can sustain this, as an unscrupulous person could fill out fake
trade-in forms and use the coupons without sending anythin... 阅读全帖 |
|
w*******y
发帖数: 60932 | 45 Per Major Nelson's Blog:
The latest Xbox LIVE Deal of the week is great news for Tom Clancy Fans.
The following content is now available for Xbox LIVE Gold Members for 50%
off:
Splinter Cell Conviction Deniable Ops: Insurgency -- 400 points
Tom Clancy's Hawx Air Supremacy -- 400 points
Tom Clancy's Hawx 2 All in One Pack -- 400 points
Tom Clancy's GRAW 2 Co-op Collection -- 400 points
Tom Clancy's GRAW 2 Co-op Collection 2 -- 400 points
Tom Clancy's Ghost Recon Advanced Warfigher Ch. 2... 阅读全帖 |
|
w*******y
发帖数: 60932 | 46 Dell XPS 17 laptop with Intel Core i7-2630QM, Win7, 8GB, 640GB 7200PRM, Full
HD 1080p display with webcam, GeForce GT 550M 1GB, Bluray, 6 cell, 2yr
service, at $999 after coupon with free shipping
Link:
http://configure.us.dell.com/dellstore/config.aspx?oc=DNDNKTw4&
Coupon code B2X9T6F5PF9DFF
IntelCore i7-2630QM
Genuine WindowsHome Premium, 64-Bit, English
2 Year Premium Hardware Service
NVIDIAGeForceGT 550M 1GB graphics with Optimus
8GB Shared Dual Channel DDR3 Memory
56 WHr 6-cell Lithium Ion... 阅读全帖 |
|
w*******y
发帖数: 60932 | 47 EXPLOSIVE ONLINE PLAY - Brand-new addictive multiplayer modes amp up the
dramatic intensity like never before.
EXTRA Content includes SCAR-H, VR ZVEZDA, Infiltration Mode, SPAS-12, SC3000
, SR-2M, MP5 -SD3, MP7A1 Machine Pistol, Proximity Mine, FAMASG2 Assault
Rifle, 3E Elite Uniform, Hunter Map: Third Echelon, VIKHR Sub-Machine Gun,
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w*******e
发帖数: 15912 | 49 http://blog.sciencenet.cn/blog-51597-748813.html
诺贝尔奖获得者号召抵制Nature, Cell and Science期刊 精选
已有 11271 次阅读 2013-12-11 01:33 |个人分类:学术与学养|系统分类:海外观察|关
键词:诺贝尔奖获得者号召抵制Nature, Cell and Science
今天海外许多媒体报道了下列新闻：今年诺贝尔医学奖获得者Randy Schekman 教授号
召抵制Nature, Cell and Science这样的“奢侈”期刊，表示他的实验室将不再投稿这
些被有些人认为是“顶尖”的学术期刊。他认为， 这些期刊误导学术界一味地追求发
表所谓的抓眼球的“时髦”的科学成果，从而导致科学家放弃对重大科学问题的持久的
思考与执着的研究， 这是对科研内界过程与基本目的的“颠倒”与“歪曲”，“科学
发表的暴政(Tyranny)”必须打破， 使其不再阻碍科学的发展。近30年来，科学界流行
起非常不良的“时尚”之风， 只要在Science、Nature之类高引期刊发表文章就能获得
更大的利益：获得基金、晋... 阅读全帖 |
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发帖数: 1 | 50 问题是，有的老板自己都没有cell。。。你还指望他能指导出五片cell？？？ |
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