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D*********t
发帖数: 370 | 2 U.S. Judge Rules Against Obama's Stem Cell Policy
By GARDINER HARRIS
Published: August 23, 2010
WASHINGTON — A federal district judge on Monday blocked President Obama's
2009 executive order that expanded embryonic stem cell research, saying it
violated a ban on federal money being used to destroy embryos.
The ruling came as a shock to scientists at the National Institutes of
Health and at universities across the country, which had viewed the Obama
administration's new policy and the grants prov |
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s******y
发帖数: 28562 | 3 Manipulating the Mouse Embryo
A laboratory Manual
Cold Spring Harbor laboratory Press
Edited by Andres Nagy etc. |
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s******y
发帖数: 28562 | 4 是的。
不过某些细胞株分泌的量不一定够多，所以如果是你第一次用那个细胞株的话，
要注意观察embryo cell的形态。 |
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o**********y
发帖数: 1041 | 5 Steptoe, P. C., and Edwards, R. G. (1978) "Birth after the reimplantation of
a human embryo". Lancet. 2: 366. |
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h*******o
发帖数: 4884 | 6 I was under the impression that perfusion is mainly to get rid of the blood
or more specifically IgGs out of the tissue so there will be much less
background in IHC.
Correct me if I am wrong
For embryos, endogenous IgGs are relatively low compared to adult tissues.
Maybe that's why it works well without perfusion. |
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f****b
发帖数: 2410 | 7 质疑曾凡一在美国的学位
呼唤方洲子
====================================
这为曾女据说本科和 MD/PHD都是在美国读的，然后就回国了，是挺奇怪的，她据说是
１９８７年来美国读的本科，来源忘了，自然"这种杂志，根本不会查你是从哪里毕业
的，你
则么写它就则么登，但上面所在单位应该是准确的，
如果能够证明她在学位上造假，应该影响很大的，
谢谢你让我们注意到这一点
Google
她应该是２０００年+/- MD/PHD毕业，可２００５年到２００７年还在upenn别人实验
室发PAPER，
http://www.med.upenn.edu/pharm/faculty/eberwine/eberwine.html
Laboratory Personnel: Fanyi Zeng
Post-doctoral Fellow
z***[email protected]
got one in ScholarlyCommons: Repository
Fanyi Zeng 2005 Gene expression during preimplantation de... 阅读全帖 |
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s**********y
发帖数: 122 | 8 1.Improved efficiency of bovine somatic cell nuclear transfer by optimizing
operational procedures.
Zhao LW, Yang XY, Guan PF, Fu J, Li H, Zhou YY, Huang SZ, Zeng YT, Zeng FY.
J Reprod Dev. 2009 Oct;55(5):542-6. Epub 2009 Jul 1.
PMID: 19571467 [PubMed - indexed for MEDLINE]Free Article
Related citations
2.Selection of in vitro produced, transgenic embryos by nested PCR for
efficient production of transgenic goats.
Huang SZ, Huang Y, Chen MJ, Zeng FY, Ren ZR, Zeng YT.
Theriogenology. 2001 Sep 1;5... 阅读全帖 |
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f****b
发帖数: 2410 | 9 质疑曾凡一在美国的学位
呼唤方洲子
evident is here
http://www.med.upenn.edu/camb/graduates.shtml
Cell & Molecular Biology Graduate Group
ZENG, FANYI 2005A 学位授予年代是２００５
网上可以查到２００５在别人实验室发表文章和２００７年
博士后得的一个奖，这都说明她在美国的学位是在２００５年得的，
Dr. Fanyi Zeng, a postdoctoral researcher in the
Eberwine lab, was awarded a 2007 PhRMA Foundation
她的美国MD 很可能是假，美国MD／PHD (指同一个MD／PHD Prgram) 可以可定是假的
她是那了一个学位在UPenn，那很可能是PhD ２００５年授予的，这和其他地方说的她
在１９９２年入的UPenn的MD／PhD是不一致的，要是真的，她应该在２０００毕业了
the following link is Upenn MD/PHD program history list... 阅读全帖 |
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h******y
发帖数: 1374 | 10 Scientists turn human skin into blood
Scientists at McMaster University have discovered how to make human blood
from adult human skin.
The discovery could mean that people needing blood for surgery, cancer
treatment or treatment of blood conditions like anemia will be able to have
blood created from a patch of their own skin to provide transfusions.
Clinical trials could begin as soon as 2012.
The researchers have also shown that the conversion is direct. Making blood
from skin does not require ... 阅读全帖 |
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l******g
发帖数: 2 | 12 Postdoc positions are available for highly motivated, independently thinking
individuals with strong interests in
protein engineering and basic molecular/cellular mechanisms.
Our lab is focused on protein design and evolution for molecular imaging,
and application to biology. In
particular, we are engineering blue and red-shifted infrared fluorescent
proteins. Part of this work was
published last year in Science. We are also engineering genetically encoded
tags for electron microscopy,
protein a... 阅读全帖 |
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m*********7
发帖数: 606 | 13 据Scopus上的信息来看，此通讯发表２年后，有关细节Establishing full-term human
pregnancies using cleaving embryos grown in vitro 才在British Journal of
Obstetrics and Gynaecology 上发表。
直至１９８３年，柳叶刀才再次发表他们俩的论文，总结１９８０年至１９８３年间试
管婴儿的出生状况。
个人感觉他们这篇通讯似乎当时不怎么受重视。这篇通讯，到今时今日，Scopus数据库
内统计才被引用５０８次，是Edwards２００多篇学术论文中被引用次数最多的一篇论
文。 |
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m*p
发帖数: 226 | 14 DNA sequence may be not the reason. All the cells in the whole organism are
derived from the same fertilized
egg: a cell. Random homologous recombination and random point mutation
happen during the development,
but these do not contributes to the organized or programmed differentiation
process in different organs, and
also does not contributes to the cell identity in different organs either.
After the fertilization, the first cell division in some if not most
organisms is asymmetric cell divisio... 阅读全帖 |
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M*****n
发帖数: 16729 | 15 i am not sure in a fish embryo the first or the second cell division is
asymetric.
correct me if i am wrong.
are
differentiation |
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x**w
发帖数: 112 | 16 1. try to get some probes as control (ntl, myoD or somehing)
2.fix the embryos with fresh prepare PFA |
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b****n
发帖数: 311 | 17 呵呵，看看薛红卫，去掉了20几篇plant cell以下的。
1: Wu GZ, Xue HW. Arabidopsis β-ketoacyl-[acyl carrier protein] synthase i
is
crucial for fatty acid synthesis and plays a role in chloroplast division
and
embryo development. Plant Cell. 2010 Nov;22(11):3726-44. Epub 2010 Nov 16.
PubMed
PMID: 21081696; PubMed Central PMCID: PMC3015132.
2: Dai C, Xue HW. Rice early flowering1, a CKI, phosphorylates DELLA protein
SLR1
to negatively regulate gibberellin signalling. EMBO J. 2010 Jun 2;29(11):
1916-27.
Epub 2010 Apr 16... 阅读全帖 |
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b****n
发帖数: 311 | 18 Kang L, Wang J, Zhang Y, Kou Z, Gao S (2009) iPS cells can support full
term development of tetraploid blastocyst-complemented embryos. Cell Stem
Cell 5(2):135-138.
这篇和周琪的nature一起证明了ips的全能性。人家也是被好多外国报刊采访过的。大
家别瞎猜了，多读多看比较靠谱。 |
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z****u
发帖数: 1007 | 19 microdissection是我们的daily work. 在dissection microsope下面解剖E10.5 mouse
embryo的first branchial arch..整个长度不超过2mm吧。然后把distal part ,
proximal part 分开，体外培养。
楼主需要的agar dish.2% agar加入碳粉让它变黑，然后autoclave之后铺petri dish.
black agar dish 背景解剖容易得多。
finescience的那些工具不错，我们一直都用它。
一定需要人带着做一遍。 |
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s***m
发帖数: 6197 | 20 Pre- and Postimplantation Development of Swine-Cloned Embryos Derived from
Fibroblasts and Bone Marrow Cells after Inhibition of Histone Deacetylases
刊物：Cellular Reprogramming (Formerly "Cloning and Stem Cells") - 12(1):85-
94;
Please send to x****[email protected]
Thanks! |
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w********u
发帖数: 5457 | 21 cell? section? embryo?
有时间还是自己做吧，公司其实也都不可靠。
出钱我帮你做也行，^_^！ |
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z****u
发帖数: 1007 | 22 ERT2 。。据说比ER 好。更强效，更特异。
tamoxifen induce的问题在于不会彻底，比如K14-Cre ERT2 在身体不同部位皮肤的诱导效
率就不一样。从30%到 60不等。所以这个诱导后更像是生成一个mosaic.当然了，有人要的就是这种mosaic KO效果。反正KO效果是dosage dependant的。另外
tamoxifen有毒性。剂量需要optimize。我目前做的一个Cre-ERT2 就遇到这个麻烦，在E18
.5打标准剂量Tamoxifen后所有embryo都会在出生后一天内死掉。 |
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s********8
发帖数: 619 | 23 大家fix embryo的时候都fix 多久啊,protocol上说是4C O/N.但是我们实验室的人告诉
我说可以leave in fix over the weekend.大家都是怎么做的?我是用来做 insitu的,
不知道时间太长了会不会overfix啊?
bow了先~~ |
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s********r
发帖数: 312 | 24 ZFN做knock-in的话大片段像2kb以上就不太好插入了，而且效率也低，做老鼠的话打那
么多embryo，还要都生出来做genotyping,都是钱啊... 鱼的我不懂，可能便宜很多吧 |
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y******8
发帖数: 1764 | 25 No only for hematopoietic system. Basically, you can screen for any
phenotype, but use soluble molecules instead of genetic methods. By this way
, you would directly get drug candidates, not just drug target candidates.
The transparency of fish embryo is overstated. If you want deal with any
late-onset disease, the adult fish is not very transparent. |
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A****A
发帖数: 16 | 26 目前的观点是，HR和NHEJ是在DNA双链断裂修复中互相竞争的pathways。如果能够有效
的抑制NHEJ,HR的频率有可能会有大幅度的提升。目前，一些labs主要通过knock out或
者knock down NHEJ中几个关键player， 比如，Ku70， Ku80 以及Ligase4，来进行验
证。比较convincing的data有Dana Carroll在果蝇的研究，以及Eric Hendrickson在
mammalian cell line中的研究。Paper 如下:
Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8703-8. Epub 2008 Jun 18.
Ku70, an essential gene, modulates the frequency of rAAV-mediated gene
targeting in human somatic cells.
Fattah FJ, Lichter NF, Fattah KR, Oh S, Hendrickson EA.
Genetics. 2009 Jul;1... 阅读全帖 |
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M*****n
发帖数: 16729 | 27 下一步就是要拿到germline
光是chimera 还发不了大paper.
其实HR肯定可以发生，就是效率太低。
俺有一个想法就是
如果直接manipulate embryos效率太低的话，可以先转一个artificial chromosome作
一个stableline.然后跟抑制NHJR的mutant line cross，然后后代筛选homologous
recombinants.
鱼能产很多卵，筛选几万个应该不难。 |
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l*h
发帖数: 4124 | 28 What is your question?
If the antibody has not been successfully used in whole mount in your lab,
you may try to cryosection the embryos for testing. |
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j*****d
发帖数: 787 | 29 //地地道道的理想主义者
而且他这篇文章不知道要得罪多少体制内 有共产党头衔的 红色科学家
http://blog.sciencenet.cn/home.php?mod=space&uid=2237
共产党员 精选
已有 8220 次阅读 2011-6-28 09:01 |系统分类:观点评述|关键词:剑桥大学 共产党员
诺贝尔奖 科学家
加入执政党的党员，原因不易单一化地叙述。
加入还在奋斗期间的党、或永远不能执政的党，其党员绝大多数是为了理念。
英国共产党（1920-1991）恐怕从来没有执政的机会。英共党员多彩多姿，如结构生物
学先驱（被人称Sage/智者的）JD Bernal（他的学生诺贝尔奖得主Hodgkin不是党员，
但她丈夫曾是）、著名遗传学家JBS Haldane、进化论学者John Maynard Smith，其中
不乏有剑桥大学、牛津大学的教授。他们入党，对自己的学术生涯有影响，可以说基本
是负面的。最简单的是，号称无政治歧视的美国，禁止共产党员入境，无论党员是英国
剑桥大学教授、是世界著名科学家、还是是美国需要征求特定领域意见的皇家学会会员
，都被这样对待。
英国著... 阅读全帖 |
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x******h
发帖数: 838 | 30 就是，打倒这批不调查的章良黑。章良再怎么，这个论文记录可以羞死大部分 wsn。86
年回国，还只混个副教授（现在网上通行说法，另有版本说仅是讲师，也许真的仅是
讲师然后很快升为副教授），试问 wsn 有几个愿意？
Regulated expression of genes encoding soybean beta-conglycinins in
transgenic plants.
Chen ZL, Naito S, Nakamura I, Beachy RN.
Dev Genet. 1989;10(2):112-22. Erratum in: Dev Genet 1989;10(4):345.
A DNA sequence element that confers seed-specific enhancement to a
constitutive promoter.
Chen ZL, Pan NS, Beachy RN.
EMBO J. 1988 Feb;7(2):297-302.
Functional analysis of regulatory elements in a ... 阅读全帖 |
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q**********0
发帖数: 335 | 32 As I known, no any commercial available for REF, you have to make by
yourself from rat. |
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p*******r
发帖数: 4048 | 35 Maybe put iPS into pig embryo |
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s******y
发帖数: 28562 | 39 对啊，而且EMBO听起来和Embryo发音相似，别人一猜就知道是生物的杂志 |
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a******p
发帖数: 414 | 40 我用B6做过，但是注射了两次embryo都没有老鼠生出来，后来用了129 就出来了。 问
了别人，说这个问题是很普遍的。 |
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c********n
发帖数: 67 | 41 Infectious disease modeling and innate immune function in zebrafish embryos.
PMID: 21951535
c*****[email protected]
Thanks very much for your kindly help! |
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a***y
发帖数: 19743 | 42 http://www.wired.com/threatlevel/2012/02/patent-troll-trial/
Michael Doyle, a low-profile Chicago biologist, claims that it was actually
he and two co-inventors who invented — and patented — the “interactive
web” before anyone else, while they were employed by the University of
California back in 1993. Doyle argues that a program he created at the UC’s
San Francisco campus, which allowed doctors to view embryos over the
nascent World Wide Web, was the first program that allowed users to interact... 阅读全帖 |
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s********r
发帖数: 312 | 43 是打embryo做transgenic mice？还是往mES里转基因？ |
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s********r
发帖数: 312 | 44 是打embryo做transgenic mice？还是往mES里转基因？ |
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l**********1
发帖数: 5204 | 45 please go to
//evol.mcmaster.ca/cgi-bin/my_wrap/brian/evoldir/PostDocs/
then search web with key word:
Droso
match three
then click just one:
Postdoctoral Researcher in Drosophila Evolutionary Genomics
Potential start dates are between April 1 and July 1, 2012. Please
indicate the earliest start date you would consider.
Feel free to contact me with any questions (jpool (at) wisc.edu).
John Pool
Assistant Professor
Laboratory of Genetics
University of Wisconsin-Madison
jpool (at )wisc.edu
----
A... 阅读全帖 |
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w***y
发帖数: 493 | 47 我现在正在尝试从zebrafish embryo的total RNA里面提取mRNA， 用的是invitrogen的
Dynabeads和他们的mRNA purification kit。 按照他们的protocol做完后跑胶发现里
面还是有挺多rRNA。上网搜了一下发现有的人建议连续做2次protocol里说的
purification。 请问大家有什么建议么？
我试了一下发现第1次纯化后获得了大概3.8%的total RNA。第二次以后有1.5%的total
RNA。我不太确定这样连续2次purification会不会损失太多mRNA。 |
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g*********5
发帖数: 2533 | 48 some extreme cases could be....in animal...
when 3 zygotes develop, too close and mix form a mosaic and develop into one
embryo..
most case in human would form with teratoma |
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m*********r
发帖数: 136 | 49 Embedding, serial sectioning and staining of zebrafish embryos using JB-4
resin
Thanks
y******[email protected] |
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g*********5
发帖数: 2533 | 50 I saw some companies have transgenic mouse embryos.
Is it easy to transfer them into female mice by the mouse facility people?
thanks. |
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