w******e
发帖数: 1187 | 1 1st of all, I actually don't do peptide aptamer myself, so don't trust
me too much:) following is the impression I got from reading.
afaik, the peptide w/ best kd is some RGD variant--there are a loooot
of variants, such as dimer/multimer of RGD, cyclic RGD, peptidomimetic
RGD, etc. It seems to me that no linear peptides has very good kd (again
from very small sample size), since peptide chain is too flexible.
circulirization/stapling is believed to increase kd by confining the
possible conforma |
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z*****m
发帖数: 34 | 2 美国本年度极为关注的一个知识产权案分析
2004年3月2日，IntegraI生命科学有限公司、Burnham研究所、Telios医药公司控告
MerckKgaA、Scripps研究所、DavidA.Cheresh博士侵犯五项专利权的案子被上诉到美国
联邦最高法院。目前，该院已经正式立案。由于该案将决定企业、科研机构、科学家在
科学研究中对专利技术进行的非授权使用是否构成专利侵权，因此它已成为美国媒体本
年度最为关注的知识产权大案之一。
本案中，三家原告拥有的五个美国专利，保护纤维粘连蛋白上的一条三肽片断。其序列
为Arg-Gly-Asp，简称为RGD肽片断。该片断容易黏附到细胞表面的avβ3受体上，诱发
更好的细胞结合，并促进细胞生长，从而促进伤口愈合，有利于血管增加分支。
Cheresh博士发现，通过阻塞avβ3受体，人们可以抑制新血管生长中的脉管发生过程来
阻止肿瘤生长。此治疗方法还可以用于治疗糖尿病性视网膜病、风湿性关节炎、牛皮癣
、肠炎等。
后来，Merck雇佣Cheresh博士利用上述机理去识别可能抑制脉管生长的候选药物。不久
，Cheresh发现，环状肽EMD66203能很... 阅读全帖 |
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w******e
发帖数: 1187 | 3 I tried to look up some kd info on RGD variant, but so far only
came up w/ this:
Modification of the amino acids
flanking the RGD sequence led to the synthesis
of EMD121974 (Cilengitide) inhibiting
aVb3 integrin binding to vitronectin with
an IC50 of 0.6 nM versus 900 nM for the
aIIbb3 integrin (Smith 2003).
I'm sure I read somewhere w/ more detailed description on peptide kd
(they are few and are b/w thou). I'll let u know when I come across
such info again.
one more thing, based on my even mor |
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w********h
发帖数: 12367 | 4 像他这样提出问题是很简单的，
别说他，以及你，
象我这样的刚入门的也马上意识到这个问题，
而且我接触的这个领域的人，人人都知道，
关键是：你能做什么？
理想总归是远大的，现实却是无奈的，
大部分人还不得赶紧做点能发表的东西毕业的毕业，找位置的找位置，
申请funding的申请funding?不要说一片森林，你找到一棵树比如RGD，
你就够牛了。。。 他Doug说了半天就他牛？他无非是不喜欢材料的人
过来混而已，然后至于他能找到什么，管他。。。我就不相信一个cell
biology的人能弄懂材料与cell function之间，材料与rgd的关系。。。 |
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j*******0
发帖数: 13 | 5 我的网站被GFW给block了，也就是说，大陆的朋友们看不到我的网站了，我能申诉么？
我的网站是 http://www.visionopen.com
目的很单纯，就是纯学术交流。
但是可能在Forum里转贴了一些比较激进的言论...
如果是关键字查询有问题，那我拿掉这些所谓激进的帖子就完了！
我很想让大陆的朋友能够浏览到我的网站...
Can anybody help?
Rgds |
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d**********0
发帖数: 13081 | 6 这个学校是天才的摇篮， 300 中占了15名。。
The Harker School, San Jose, CA
Dixit, Shikhar, 17
Immunomodulation by Human Retinal Pigment Epithelial Cell Line ARPE-19
Jin, Andrew, 17
Shedding Light on Human Evolution: Machine Learning Algorithms for
Systematic
Genome-wide Discovery and Characterization of Adaptive Mutations
Kuditipudi, Rohith, 18
Network-based Integration of High Throughput Gene Expression and Methylation
Data
Lin, David, 17
Physical Properties and Evolution of
Lin, David, 17
Physical Properties an... 阅读全帖 |
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s**p
发帖数: 207 | 7 I bought ICS prime plan rather than plan A
with 20$ more on half a year. I eliminate the 1500 maximum inpatient
hospital services.
when you are in hospital, $1500 is really not enough.
I am not a professional. This is just a suggestion.
best rgds. |
|
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h****9
发帖数: 381 | 9 ＊＊＊ SALE或COUPON专贴，请JMs将有关信息贴这个专贴里，以方便大家查找 ＊＊＊
＊＊＊ 请JMs存下这个贴子的链接，我会尽量每周都来更新，如果大家看不到这个贴子，我更新就没有意义了。 ＊＊＊
只是想跟JMs说明一下，为了方便大家查找，我答应版版MM帮助收集和整理我所
知道的以及JMs提供的COUPON/DEAL信息，我会尽量及时更新coupon专贴的主贴，
也欢迎更多JM将coupon贴在这个贴子里，在此一并感谢所有提供COUPON/DEAL
信息与大家分享的JMs，并感谢在COUPON专贴出问题时给予我很大支持与帮助的steedzhu、BelugaWhale及brightsmoo几位版版MM。
除了某些 BRU/TRU COUPON可能是扫描件以外，其它基本上都是printable coupons，请需要的JM用链接打印啊。JMs可以在文摘区找到更多coupon信息
包括交换等信息(请从首页找起)。
★ 有JM问起，觉得这个贴子太长了，慢的原因可能是图片太多，我将图片显示出来是想方便JMs很快找到自己想要的DEALS(我可以将图片略去，如果更好的话)。简介一下主贴的内容啊... 阅读全帖 |
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o******6
发帖数: 9458 | 10 找到加入kohls rewards的链接了https://kohlsrewards.com/cp/k_landing.aspx?RGD=
ceca1fb2-28d6-481a-b032-191938ba5f4a
填完我这的ZIP Code，显示We're sorry, Kohl's Rewards is not yet available
in your area. Check back soon. We're expanding quickly! |
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g******g
发帖数: 1664 | 11 放狗搜了下
现在才试点
全美只有100个店能enroll
https://kohlsrewards.com/CP/k_enroll_eligibility.aspx?RGD=ceca1fb2-28d6-481a
-b032-191938ba5f4a
Kohl's Rewards
In order to improve the customer experience and offer even more savings, Koh
l’s is currently testing the Kohl’s Rewards Program in approximately 100 s
tores nationwide. The program provides all customers an easy way to earn spe
cial offers throughout the year. Due to the competitive nature of our busine
ss, Kohl’s does not disclose specific details relating... 阅读全帖 |
|
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z****u
发帖数: 305 | 13 节选自环球财经（www.chineseworldnet.com）网站，有效期至当前，罗列所有到今天
（2011年3月18日）开盘为止创52周新高的股票，特此存档！
# 公司名稱 (代號) 開市價 最高價 最低價 收市價 成交量
1 PEABODY ENERGY (NYSE:BTU) 69.07 70.84 68 70.12 8,576,640
2 CAPSTONE TURBINE (NASDAQ:CPST) 1.85 1.88 1.76 1.77 7,400,432
3 CONSOL ENERGY (NYSE:CNX) 53.57 55.49 52.67 55.33 6,972,713
4 CSX (NYSE:CSX) 74.98 77.24 74.48 75.72 5,327,296
5 HECKMANN (NYSE:HEK) 6.18 6.35 6.12 6.21 4,064,357
6 WESTERN FOREST PRODUCTS (TSX:WEF) 0.96 1.23 0.96 1.18 3,921,072
7 AMERICAN CAPITAL AGENCY (NASDAQ:... 阅读全帖 |
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d*******u
发帖数: 5337 | 14 Hi reviewers,
I wish you all have a happy new year.
Please check the following manuscripts to Biomedicine and pharmacotherapy.
If you are interested in reviewing these manuscripts, send your background
and Ms #. Feel free to pass this information to your friends and collogues.
Best regards,
xxxx
Executive editor
Biomedicine and Pharmacotherapy
1. Antiproliferative activity of Corylus avellana L. extracts against
human cancer cell lines
2. Poloxamer Surface modified Trimethyl Chitosan Nano... 阅读全帖 |
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u***r
发帖数: 300 | 15 The effect of RGD-peptide coated TiO2 nanotubes on the attachment,
proliferation and functionality of bone related cells
影响因子1点几纳米材料小杂志，
站内发信，留下姓名，单位， Email，简单介绍一下研究方向。
尽力推荐 |
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s*******8
发帖数: 24 | 16 您好！
关于密封件：
我有这样的工厂。我需要更多的信息。 能否发电给我？或者在MSN上上洽谈？
我MSN:g************[email protected]
Thanaks！
B.rgds/George |
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l*****e
发帖数: 47 | 17 I agree with you.
But can you tell me how much time you saved by abbreviating "you" and "
people"?
That goes with with the rest of your WSNs who use "rgds", "thx", ... Is that
supposed to show that you're well versed in the English language?
poor
to
that
bathroom
to |
|
s**a
发帖数: 8648 | 18 一个朋友发来的信息，新泽西附近的朋友可以去打球。
Hi,
I am the coordinator of Friendly Badminton in Hoboken, NJ. Could
you guys announce our tournament to your members, please? I attached
the
registration form in pdf file.
For more infromation, please check our website:
www.Friendlybadminton.com.
Thank you for your cooperation.
Rgds,
Kim Fung
Dear all,
As may you know, we're delayed to make our Annual Friendly Badminton
Tourname |
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c****i
发帖数: 2635 | 19 Dear Edward,
Thanks for the greeting. Kindly find the reply you sought below.
Rgds,
Xi
*^皿^*~~`
1917 |
|
c****i
发帖数: 2635 | 20 Dear Edward,
Thanks for the greeting. Kindly find the reply you sought below.
Rgds,
Xi
*^皿^*~~`
1917 |
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m*******n
发帖数: 519 | 21 下面这个链接说X-T2 追焦连D600都赶不上，室内拍娃吃力，不晓得版上用xpro-2的感
觉如何？
https://www.dpreview.com/forums/thread/4080598
“X-T2 vs D750 AF-C
1 day ago
There will likely be a lot who will contradict me but this is my experience.
....
I had a Nikon D600 and had excellent AF-C experiences, it was easy to have
clean shot of my small kids. (Are you using a fast enough shutter speed?)
I then moved to Fuji X-E2 with fastests lenses, X100S, X100T and AF-C was
always a nightmare.... impossible to have a clean shot of my kids wi... 阅读全帖 |
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j*******0
发帖数: 13 | 22 呵呵...真事儿假事儿啊？
如果是真的，忍了吧，真不好！
Rgds |
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f*******a
发帖数: 2662 | 23 感谢你的意见
即日起,我停止在此贴文
对到此欣赏文章的各位朋友说一声抱歉
对感到灌水不便的各位朋友说一声抱歉
Rgds,
F |
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e***n
发帖数: 5 | 24 Hi,is there any 89 grade guy of UESTC in USA now?Please
contact me with e*********[email protected].
Here is an old friend.
Rgds! |
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|
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f*******a
发帖数: 2662 | 27 轮到我了.
版面上的文章读了两三遍,精华区一直在更新中.
技术版不象8区那些垃圾版,需要版务的耐心,细心积累.希望后来的
版务也是凭着热爱才来为大家服务的.
各种雕虫小技都没有漏掉.没有在精华区的就一定被m在版面上了.
版面帖子留7天.
上任之初严格地执行了"与盗版有关封30天""广告封30天"的政策.
希望大家理解.
感谢各位网友和前任版务们的共同努力.
大家灌的开心!
Rgds,
Forestsea
辞职自2004.01.31起生效 |
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s**p
发帖数: 207 | 28 I tried to print a .doc file generate by MS word 2000 to postscript
file
I tried a lot of setting, I can always view the PS in unix. but
I cannot view it under unix, and not under linux either.
And I can print only part of it. only several pages.
I used generic postscript printer.
Any trick there?
Best Rgds. |
|
s******y
发帖数: 28562 | 29 Thanks!
The reason people are interested in RGD peptide is because it mimics the
extracellular matrix and binds to the integrins, I believe.
I actually don't really care what kind of peptide it is. However I am kind
of planning to do some pilot experiments by myself and I prefer to use
something that can be handled easily. Therefore, I would like to have
something that is sovlable and cytoplasmic (which will be easy for
expression and require minimal posttranslational modification). While the
ex |
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w******e
发帖数: 1187 | 30 比如RGD peptide derivative能coat到ELISA plate上做capturing agent吗？
多谢！ |
|
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s******n
发帖数: 189 | 32 Name; MicroPET imaging of breast cancer alpha-v integrin expression with
64Cu-labeled dimeric RGD peptides.
谢谢！！！ |
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j*******0
发帖数: 13 | 33 RT:
Any suggestions on Handy Open Source Software for C to VHDL/Verilog ?
Rgds
Pei |
|
m***n
发帖数: 266 | 34 就是因为我现在既发不了paper,也拿不到位置，所以就只有概括性的
胡侃一通拉:-)
Doug是化工的背景，材料还是懂地...Cell biology的会请他来给开
题报告，确实有意思。
发信人: wonderlich (左岸，遁去), 信区: Macromolecules
标 题: Re: 本版做生物相关研究的注意了
发信站: BBS 未名空间站 (Sat Jul 16 02:19:09 2005)
像他这样提出问题是很简单的，
别说他，以及你，
象我这样的刚入门的也马上意识到这个问题，
而且我接触的这个领域的人，人人都知道，
关键是：你能做什么？
理想总归是远大的，现实却是无奈的，
大部分人还不得赶紧做点能发表的东西毕业的毕业，找位置的找位置，
申请funding的申请funding?不要说一片森林，你找到一棵树比如RGD，
你就够牛了。。。 他Doug说了半天就他牛？他无非是不喜欢材料的人
过来混而已，然后至于他能找到什么，管他。。。我就不相信一个cell |
|
s*****i
发帖数: 146 | 35 看你作什么细胞了吧，有的细胞是希望spread的，有的就是要保持圆形才好。不是说
spread一定好。你的gel materials也有关系，加点RGD什么的可以促进attach的 |
|
s**p
发帖数: 207 | 36 Hi
我同时也发在google group上了，所以就不想翻译了，大家帮忙看看吧
I have a full rank matrix A (n-by-n), and a mapping matrix B (B is not
square, but m-by-n with m
Then I have a matrix C = B A B.', C (m-by-m) is also full rank.
I have inv(A) and I want to know inv(C). Any efficient way of doing
it?
Rgds
I can make B square and full rank by fill in some independant rows, so
I get another matrix D, where C is part of D. D=[ C X; X X], X is some
non-zeros block. Then I might be able to find inv(C), am I on the
right tra |
|
o****e
发帖数: 1011 | 37 那些internalizing RGD (iRGD)或其他Cell-penetrating peptide都不顶用吗？
我是说，如果用这些peptide帮忙的话。（这些peptide其实“没用”？） |
|
o****e
发帖数: 1011 | 38 那些internalizing RGD (iRGD)或其他Cell-penetrating peptide都不顶用吗？
我是说，如果用这些peptide帮忙的话。（这些peptide其实“没用”？） |
|
f*******a
发帖数: 2662 | 39 【 以下文字转载自 Software 讨论区 】
【 原文由 forestsea 所发表 】
轮到我了.
版面上的文章读了两三遍,精华区一直在更新中.
技术版不象8区那些垃圾版,需要版务的耐心,细心积累.希望后来的
版务也是凭着热爱才来为大家服务的.
各种雕虫小技都没有漏掉.没有在精华区的就一定被m在版面上了.
版面帖子留7天.
上任之初严格地执行了"与盗版有关封30天""广告封30天"的政策.
希望大家理解.
感谢各位网友和前任版务们的共同努力.
大家灌的开心!
Rgds,
Forestsea
辞职自2004.01.31起生效 |
|
|
