We use T7 promoter for our vector system. We have been very successful to
produce the recombinant protein for bioconversion. Usually we induce the
cell with IPTG for 3-4 hours before bioconversion. But this time I induce
the cell for about 12 hours and I didn't get any products. The cell grows
well and seems normal. What's the consequence if you induce the cell too
long with IPTG? Does this matter with my expression results? Thank you!
