j*****a
发帖数: 658 | 1 I need do IP and 1st antibody is polyclonal. Can somebody recommendate a
company with good proteinA-coupled sepharose beads? I will order soon.. GE
health has protein A sepharose CL-4B seems veryyyyy expensive, they only
have 25ml size which is ~$1395. Can someone recommendate another one with
good quality and not so expensive? Thank you guys very much. | j*****a
发帖数: 658 | 2 GUys,,, help~~~~~
【在 j*****a 的大作中提到】
: I need do IP and 1st antibody is polyclonal. Can somebody recommendate a
: company with good proteinA-coupled sepharose beads? I will order soon.. GE
: health has protein A sepharose CL-4B seems veryyyyy expensive, they only
: have 25ml size which is ~$1395. Can someone recommendate another one with
: good quality and not so expensive? Thank you guys very much.
| c*********r
发帖数: 1312 | 3 我只用过Pierce的Protein A/G Agarose，所以不好比较是否便宜。^_^
http://www.piercenet.com/Products/Browse.cfm?fldID=01010321
你做IP 需要25ml吗？一个反应你用多少settled beads？ | j*****a
发帖数: 658 | 4 10-20ul/reaction, right? Thank you for sharing your info. No, I don't need
25ml, but I know GE health's beads are very good, but they only have 25ml
size..
【在 c*********r 的大作中提到】
: 我只用过Pierce的Protein A/G Agarose，所以不好比较是否便宜。^_^
: http://www.piercenet.com/Products/Browse.cfm?fldID=01010321
: 你做IP 需要25ml吗？一个反应你用多少settled beads？
| c*********r
发帖数: 1312 | 5 差不多吧，我用10到50 ul settled resin。
need
【在 j*****a 的大作中提到】
: 10-20ul/reaction, right? Thank you for sharing your info. No, I don't need
: 25ml, but I know GE health's beads are very good, but they only have 25ml
: size..
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