w******e
发帖数: 1187 | 1 http://www.nature.com/nbt/journal/v29/n6/full/nbt.1856.html
phage display是好东西。。。and又见next-gen seq in directed
evolution。。。well只做一个round的话不知还该不该叫evolution lol
不过这篇paper的pos control够单薄的。
Immune responses targeting self-proteins (autoantigens) can lead to a
variety
of autoimmune diseases. Identification of these antigens is important for
both diagnostic and therapeutic reasons. However, current approaches to
characterize autoantigens have, in most cases, met only with limited success
. Here we present a synthetic representation of the complete human proteome
, the T7 peptidome phage display library (T7-Pep), and demonstrate its
application
to autoantigen discovery. T7-Pep is composed of >413,000 36-residue,
overlapping
peptides that cover all open reading frames in the human genome, and can
be analyzed using high-throughput DNA sequencing. We developed a phage
immunoprecipitation
sequencing (PhIP-Seq) methodology to identify known and previously
unreported
autoantibodies contained in the spinal fluid of three individuals with
paraneoplastic
neurological syndromes. We also show how T7-Pep can be used more generally
to identify peptide-protein interactions, suggesting the broader utility
of our approach for proteomic research. | s******9
发帖数: 283 | 2 合成T7-Pep要花多少钱？如果全由公司做。
success
proteome
【在 w******e 的大作中提到】
: http://www.nature.com/nbt/journal/v29/n6/full/nbt.1856.html
: phage display是好东西。。。and又见next-gen seq in directed
: evolution。。。well只做一个round的话不知还该不该叫evolution lol
: 不过这篇paper的pos control够单薄的。
: Immune responses targeting self-proteins (autoantigens) can lead to a
: variety
: of autoimmune diseases. Identification of these antigens is important for
: both diagnostic and therapeutic reasons. However, current approaches to
: characterize autoantigens have, in most cases, met only with limited success
: . Here we present a synthetic representation of the complete human proteome
| w******e
发帖数: 1187 | 3 you should ask the author hehe
合成T7-Pep要花多少钱？如果全由公司做。
success
proteome
【在 s******9 的大作中提到】
: 合成T7-Pep要花多少钱？如果全由公司做。
:
: success
: proteome
| s******s
发帖数: 13035 | 4 没概念。我估计$1k+一个chip, 一共几万刀？
【在 s******9 的大作中提到】
: 合成T7-Pep要花多少钱？如果全由公司做。
:
: success
: proteome
|
|
