a*******g 发帖数: 33 | 1 Hello. I work with a line of myeloid cell specific gene KO mice recombined
by LysM-Cre. Boss would like to know the KO efficiency in adipose
macrophages. In stromal vascular cells from adipose tissue, CD11b magnetic
beads were used to seperate macrophages. Surprisingly, the CD11b negative
group showed a pure monocyte/macrophage group judged from morphology in Diff
quick stained slides, while the positive population included more
neutrophils.Anyone has similar adipose tissue macrophage seperation
experience to share? Thanks. | b*******n 发帖数: 605 | 2 Is this related to your KO? |
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