x****1
发帖数: 1410 | 1 Knockout 一个基因后，Macrophage 的CD11b上升，CD11c, CD64, and EpCam下降。有
啥意义或者后果。 | p**t
发帖数: 160 | 2 KO mice?
You should also check other myeloid cell populations, e.g. DC, MDSC, even
CDP, MDP. Absolute numbers and percentages.
As for macrophage, did you check F4/80, MerTk, MHCII, CD40, CD80, CD86, PD-
L1, PD-L2, morphology, and phagocytosis?
And, the phenotypic change is generally on all lymphoid organ and tissue
macrophage subsets?
【在 x****1 的大作中提到】
: Knockout 一个基因后，Macrophage 的CD11b上升，CD11c, CD64, and EpCam下降。有
: 啥意义或者后果。
| x****1
发帖数: 1410 | 3 谢谢您的建议，很有用。
KO mice
已经check了其他myloid cells了， absolute #和percentage都check了。
F4/80 MHCII CD64， CD11b， CD11c， Ly-6G， Siglee-F, 都check了。其他的没做。
phagocytosis没做
是只有tissue macrophage 有变化。
【在 p**t 的大作中提到】
: KO mice?
: You should also check other myeloid cell populations, e.g. DC, MDSC, even
: CDP, MDP. Absolute numbers and percentages.
: As for macrophage, did you check F4/80, MerTk, MHCII, CD40, CD80, CD86, PD-
: L1, PD-L2, morphology, and phagocytosis?
: And, the phenotypic change is generally on all lymphoid organ and tissue
: macrophage subsets?
| z*******6
发帖数: 679 | 4 interesting... monocytes neutrophils cd11b都没有变化？... 只有tissue
macrophage有变化？你说的是resident tissue macrophage还是challenge以后的
tissue macrophage？你做的是什么tissue的macrophage... 可以看看CD62L有没有变低
，也是一个相当敏感的marker... | x****1
发帖数: 1410 | 5 没challenging。就是knockout一个基因。Alveolar Macrophage.我不做免疫，刚到这
个实验室，分析他们以前做的data，想找个project。
在 zhangn516 (zhangn516) 的大作中提到: 】 | m*******y
发帖数: 141 | 6 M1 and M2 macrophage?
【在 x****1 的大作中提到】
: 没challenging。就是knockout一个基因。Alveolar Macrophage.我不做免疫，刚到这
: 个实验室，分析他们以前做的data，想找个project。
: 在 zhangn516 (zhangn516) 的大作中提到: 】
| p**t
发帖数: 160 | 7 Please check this paper
http://jem.rupress.org/content/210/10/1977.abstract
and also other related papers from Bart N. Lambrecht.
The phenotypic change of alveolar macrophage can come from deficiency during
develpoment.
You'd better find out a disease model in which alveolar macrophages are
important to see whether there is functional deficiency.
This paper may also be useful for you.
http://jem.rupress.org/content/210/4/775.full
【在 x****1 的大作中提到】
: 谢谢您的建议，很有用。
: KO mice
: 已经check了其他myloid cells了， absolute #和percentage都check了。
: F4/80 MHCII CD64， CD11b， CD11c， Ly-6G， Siglee-F, 都check了。其他的没做。
: phagocytosis没做
: 是只有tissue macrophage 有变化。
| x****1
发帖数: 1410 | 8 非常有用的信息
谢了。
during
【在 p**t 的大作中提到】
: Please check this paper
: http://jem.rupress.org/content/210/10/1977.abstract
: and also other related papers from Bart N. Lambrecht.
: The phenotypic change of alveolar macrophage can come from deficiency during
: develpoment.
: You'd better find out a disease model in which alveolar macrophages are
: important to see whether there is functional deficiency.
: This paper may also be useful for you.
: http://jem.rupress.org/content/210/4/775.full
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