G********e
发帖数: 528 | 1 I'd like to ask if it is possible to do so.
For example, PFA fixation, FACS sorting for specific cell population, then
lyse protein for western.
The reason not doing live cell FACS is lack of the cell surface marker for
the specific cells I'm interested in. I have to do fixation/permeablization
and intracellular marker for cell purification. But after that, it seems
very hard to get protein extract from fixed cells. Can I use mild fixation?
Please let me know your experience, if any. | s******s
发帖数: 13035 | 2 ChIP里面也有de-crosslink，试一下这些条件？
permeablization
?
【在 G********e 的大作中提到】
: I'd like to ask if it is possible to do so.
: For example, PFA fixation, FACS sorting for specific cell population, then
: lyse protein for western.
: The reason not doing live cell FACS is lack of the cell surface marker for
: the specific cells I'm interested in. I have to do fixation/permeablization
: and intracellular marker for cell purification. But after that, it seems
: very hard to get protein extract from fixed cells. Can I use mild fixation?
: Please let me know your experience, if any.
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