m**p
发帖数: 202 | 1 imaging方法除外
fluorometer? Quibit? 酶标仪? 最敏感的仪器大概需要最少多少个荧光分子能检测出
来？(solution里面测) |
e****s
发帖数: 1125 | 2 相教于仪器，标记何种荧光分子对灵敏度的影响更大。
【在 m**p 的大作中提到】
: imaging方法除外
: fluorometer? Quibit? 酶标仪? 最敏感的仪器大概需要最少多少个荧光分子能检测出
: 来？(solution里面测)
|
g*****s
发帖数: 1016 | |
m**p
发帖数: 202 | 4 同样的荧光标记和分子数量条件下呢？
【在 e****s 的大作中提到】
: 相教于仪器，标记何种荧光分子对灵敏度的影响更大。
|
A******y
发帖数: 2041 | 5 Luciferase is literally photon measurements. A lot of modern fluorometers (
i.e. plate readers) has the function to read luminescence. All you have to
do is look at their minimum detection range for the instruments. However, I
can guarantee you that more expensive ones will be better with exception of
instruments from molecular devices (old tech and still charge an arm and a
leg). |
c*******0
发帖数: 190 | 6 It depends on the optical instrumentation, illumination efficiency and
detector quantum efficiency. In other words, there is no most "sensitive"
one but the most suitable one according to budget limit and experiment
design. |
