i********i
发帖数: 329 | 1 一个IF>5的杂志。如果有人有兴趣的话，能否给我发个站内信，附上自己的CV，
research interest和official email address。这样editor office能把你的信息加到
他们的database里面。
之前三个review的机会分别给了raulan, pigtofly和cmwang。所以这次不好意思会转给
别人了哈。
谢谢！ | w*****t
发帖数: 179 | 2 您好，不知道我的背景能否审到这片文章。我曾经审过一些小杂志，希望能够对我争取
这次机会有所帮助。详细的CV和研究背景如下。非常感谢您的考虑。我的工作邮箱：
z****[email protected]
好像CV在这里粘贴很难看，不好意思。前一段时我的CV，后一段我的较详细的研究经历
。如有需要我更详细的背景情况，请跟我发信。
1
CURRICULUM VITAE
Full Name: Zhimin Wang
Address: Allegheny-Singer Research Institute, West Penn Allegheny, Health
System, South Tower, 320 East North Avenue, Pittsburgh, PA 15212-4772
Phone: 412-359-6153 (Office)
412-737-3777 (Cell)
E-mail: z****[email protected] w************[email protected]
EDUCATION
September, 2001- May, 2005 Ph.D., Molecular Microbiology.
State Key Laboratory of Virology, School of Life Science, Wuhan University,
P.R.China
September, 2000- July, 2001 Senior Researcher
New Antibiotic Screening Group, Wuhan Kernel Bio-pesticide Co., Ltd, Wuhan,
P.R.China
September, 1997- July, 2000 Master of Science, Biotechnology
Genetic Research Division, School of Life Science, Central China Normal
University,
Wuhan, P.R.China
September, 1993- July, 1997 Bachelor of Biology Education
School of Life Science, Central China Normal University, Wuhan, P.R.China
RESEARCH EXPERIENCE
July, 2011-Now, Senior Research Associate, Gene Therapy
Allegheny-Singer Research Institute, West Penn Allegheny Health System,
Pittsburgh, PA.
September, 2009- July, 2011, Postdoctoral Associate, Molecular Biology
Department of Urology and UF Prostate Disease Center, University of Florida,
Gainesville, FL.
June, 2005- September, 2009, Postdoctoral Associate, Molecular Biology
Department of Pathology, Georgia Health Sciences University (Former Medical
College
of Georgia), Augusta, GA
2
PUBLICATIONS
1. Wang Z, Kim J, Frilot N, Daaka Y. Dynamin2 S-nitrosylation regulates
adenovirus type 5 infection of epithelial cells. Journal of General Virology
2012
Jul 12.
2. Wang Z, Humphrey C, Frilot N, Wang G, Nie Z, Moniri NH, Daaka Y.
Dynamin2- and endothelial nitric oxide synthase-regulated invasion of
bladder
epithelial cells by uropathogenic Escherichia coli. Journal of Cell biology
2011
Jan 10; 192(1): 101-10.
3. Kim JI, Chakraborty P, Wang Z, Daaka Y. G-protein coupled receptor kinase
5
regulates prostate tumor growth. Journal of Urology 2012 Jan 187 (1): 322-29.
4. Zhu Y, Wu Y, Kim JI, Wang Z, Daaka Y, Nie Z. Arf GTPase-activating
protein
AGAP2 regulates focal adhesion kinase activity and focal adhesion remodeling.
Journal of Biological Chemistry 2009 May 15; 284 (20):13489-96.
5. L.Hu, Wang Z, Hu C, Liu X, Yao L, Li W, Qi Y. Inhibition of measles virus
multiplication in cell culture by RNA intereference. Acta Virological 2005;
49
(4): 227-31.
6. Wang Z, Hu L, Yi G, Xu H, Qi Y, Yao L. ORF390 of white spot syndrome
virus
genome is identified as a novel anti-apoptosis gene. Biochemical and
Biophysical
Research Communications 2004, 352: 899-907.
7. Liu Y, Dong W, Chen L, Xiang R, Xiao H, De G, Wang Z, Qi Y. BCL10
Mediates Lipopolysaccharide/Toll-like Receptor-4 signaling through
interaction
with Pellino2. Journal of Biological Chemistry 2004 Sep 3; 279 (36): 37436-
44.
8. Yi G, Wang Z,Qi Y, Yao L, Qian J, Hu L, Vp28 of Shrimp White Spot
Syndrome
Virus Is Involved in attachment and penetration to shrimp cells. Journal of
Biochemistry and Molecular Biology 2004 Nov 30; 37(6): 726-34.
9. Yi G，Qian J，Wang Z, Qi Y. A phage－displayed peptide can inhibit
infection
by white spot syndrome virus of shrimp. Journal of General Virology 2003 Sep;
84(Pt 9): 2545-53.
POSTER PRESENTATIONS
3
1. America Society Gene and Cell therapy 15th annual meeting (May, 2012):
Hydrodynamic gene transfer of a Cox-1/Prostacyclin synthase fusion protein
results in
endogenousproduction of supra-therapeutic levels of prostacyclin in rats.
2. UF COM Celebration of Research (March 2011): Dynamin2- and endothelial
nitric oxide synthase-regulated invasion of bladder epithelial cells by
uropathogenic
Escherichia coli.
SCENTIFIC MEMBERSHIP
Associate Member of American Society of Gene and Cell Therapy (ASGCT).
PEER REVIEWER
Journals: Wuhan University Journal of Natural Sciences
Amino Acid and Biotic Resource
Indian Journal of Biochemistry & Biophysics
Sciencepaper Online
Molecular Biology Reports
The Journal of Bioscience and Medicine
Research Experience
July, 2011- present
Senior Research Associate: Allgheny-Singer Research Institute, West Penn
Health
System, Pennsylvania, U.S.A
Major Project 1: Salivary gland-based gene therapy for lysosomal storage
diseases
Key achivements:
Fabry disease is a member of the family of lysosomal storage diseases, a
group of
monogenic disorders characterized by deficiency or lack of a class of
enzymes such as
apha-Galactosidase A in Fabry disease (GLA). Recent studies demonstrated
that an
intrinsic endocrine pathway is present in salivary glands and this distinct
pathway has
been shown to be capable of robust synthesis and secretion of on-regulated
gene products
into the vascular space. We generate the AAV vector containing the sequence
for human
GLA delivered to the salivary glands in alpha-Gal A deficient “Fabry” mice
. The specific
aims involve examination the organ biodistribution of the enzyme and
optimization of
dosing and immune physiology to achieve long-term expression of the enzyme.
Major Project 2: Adenovirus gene transfer of anti-biofilm peptides to the
salivary gland
Key Achivements:Gene transfer to the salivary glands presents the
opportunity to achieve
endogenous production and salivary secretion of peptide therapeutics.
Endogenous
production overcomes many of the challenges that have thus far limited the
utility of
emerging anti-biofilm peptide molecules for targeting individual bad actors
in the
periodontal flora. We generate the recombinant adenovirus containing the LL-
37
antimicrobial peptide and delivered to the salivary glands in rodent model.
The aim is to
achieve a steady-state, sustained concentration of the therapeutic in the
saliva and
evaluate the antio-biofilm potency of saliva from treated mice.
Major Project 3: ultrasound-assisted Aquaporin-1 (AQP1) gene therapy for
functional
restoration of salivary gland.
Key achivements: We have recently reported the development of an ultrasound-
assisted
gene transfer (UAGT) method in the salivary gland. This technique enables
transient,
non-invasive, non-viral gene transfer to the salivary gland using "off the
shelf" FDAapproved
microbubbles and clinical ultrasonography equipment. By using next
generation mini circle non-viral vectors, we will scale our UAGT technique
from mice to
swine, perform AQP1 gene therapy using UAGT in the salivary glands of rats
and swine
rendered xerostomic by ionizing radiation and characterize the proteome of
saliva
produced by treated animals with and withous partial sparing of the salivary
gland by
intensity-modulated radiation (IMRT).
August, 2009- June, 2011
Postdoctoral Associate: Molecular Biology, Department of Urology, University
of
Florida, Gainesville, FL U.S.A (Moved with Supervisor from Georgia Health
Sciences
University to University of Florida)
June, 2005- July, 2009
Postdoctoral Associate: Department of Pathology, Georgia Health Sciences
University
Major Project 1: Dynamin2- and endothelial nitric oxide synthase-regulated
invasion of
bladder epithelial cells by uropathogenic Escherichia coli.
Key Achivements: Invasion of bladder epithelial cells by uropathogenic
Escherichia coli
(UPEC) contributes to antibiotic-resistant and recurrent urinary tract
infections (UTIs),
but this process is incompletely understood. In our work, we provide
evidence that the
large guanosine triphosphatase dynamin2 and its partner, endothelial nitric
oxide (NO)
synthase (NOS [eNOS]), mediate bacterial entry.
Related Publication: Wang Z, Humphrey C, Frilot N, Wang G, Nie Z, Moniri NH,
Daaka Y. Dynamin2-
and endothelial nitric oxide synthase-regulated invasion of bladder
epithelial cells by uropathogenic
Escherichia coli. Journal of Cell biology 2011 Jan 10; 192(1): 101-10.
Major Project 2: Dynamin2 S-nitrosylation regulates adenovirus type 5
infection of
epithelial cells.
Key Achivements: Dynamin2 is a large GTPase that regulates vesicle
trafficking, and the
GTPase activity of dynamin2 is required for the multistep process of
adenovirus infection.
Activity of dynamin2 may be regulated by post-translational phosphorylation
and Snitrosylation
modifications. In our study, we demonstrate a role for dynamin2
Snitrosylation
in adenovirus infection of epithelial cells. The results support the
hypothesis
that dynamin2 S-nitrosylation following eNOS activation facilitates
adenovirus infection
of host epithelial cells.
Related Publication: Wang Z, Kim J, Frilot N, Daaka Y. Dynamin2 S-
nitrosylation regulates
adenovirus type 5 infection of epithelial cells. Journal of General Virology
2012 Jul 12.
September, 2001- May, 2005
Research Assistant (Ph.D candidate): The College of Life Science, Wuhan
University,
Hubei Province, P.R.China
Major Project 1: Identify novel anti-apoptosis gene in white spot syndrome
virus
(WSSV)
Key Achivements: Apoptosis serves as an important defense strategy employed
by host
cells against viral invasion. Many viruses contain the anti-apoptotic genes
to block the
defense-by-death response of host cells. Using this marker rescue assay, we
identified
the function of ORF in WSSV is a kind of apoptotic suppressor like P35 in
AcMNPV.
Related publication: Wang Z, Hu L, Yi G, Xu H, Qi Y, Yao L. ORF390 of white
spot syndrome virus
genome is identified as a novel anti-apoptosis gene. Biochemical and
Biophysical Research
Communications 2004, 352: 899-907.
Major Project 2: Inhibition of Measles virus multiplication in cell culture
by RNA
interference
Key Achivements: Measles takes a toll of one million people a year worldwide,
especially in developing countries and novel approaches to this problem are
needed. In
this study, we investigated the possibility of inhibition of Measles virus (
MV) infection
through RNA interference (RNAi), namely the interference with the second but
main
receptor of MV, signaling lymphocyte activation molecule (SLAM), in B95-8
cell line.
The results suggest that RNAi can silence SLAM expression and inhibit the MV
infection
of host cells.
Related publication: L.Hu, Wang Z, Hu C, Liu X, Yao L, Li W, Qi Y.
Inhibition of measles virus
multiplication in cell culture by RNA intereference. Acta Virological 2005;
49 (4): 227-31.
EXPERIMENTAL SKILLS
Molecular Biology
DNA Recombination: Vector Construction, Site-directed Mutagenesis
Immunological techniques: Immunoflourescence, Immunohistochemistry,
FACS,
ELISA, MTT
Study of protein-protein interactions: GST Pull-down, Co-IP , Yeast
two hybrid
Cell fraction extraction: Membrane and Nuclear protein extraction
in vitro transcription : RT-PCR, Real-time PCR
Imaging techniques: Confocal Microscopy, Flourescence Microscopy.
Protein Nitrosylation: Biotin-switch Assay
Cell Biology
Cell Culture, Primary Cells Isolation (Endothelial Cells, Shrimp
primary
lymphocyte cell culture, macrophages)
Cell Migration, Cell Invasion, Gelatin Zymography
Lenti-virus transduction Transfection, Electroporation, RNA
Interference
Cell Cycle, Apoptosis
Generating Recombinant Adenovirus and Adeno-associate virus
Ultrasound-assisted gene transfer (UAGT), Hydrodynamic gene
transfer
【在 i********i 的大作中提到】
: 一个IF>5的杂志。如果有人有兴趣的话，能否给我发个站内信，附上自己的CV，
: research interest和official email address。这样editor office能把你的信息加到
: 他们的database里面。
: 之前三个review的机会分别给了raulan, pigtofly和cmwang。所以这次不好意思会转给
: 别人了哈。
: 谢谢！
| i********i
发帖数: 329 | 3 这次这个已经refer掉了，不好意思。能否发一封CV在我邮箱（包括email address），
这样我再有机会会主动发邮件给你。
谢谢了。
【在 w*****t 的大作中提到】
: 您好，不知道我的背景能否审到这片文章。我曾经审过一些小杂志，希望能够对我争取
: 这次机会有所帮助。详细的CV和研究背景如下。非常感谢您的考虑。我的工作邮箱：
: z****[email protected]
: 好像CV在这里粘贴很难看，不好意思。前一段时我的CV，后一段我的较详细的研究经历
: 。如有需要我更详细的背景情况，请跟我发信。
: 1
: CURRICULUM VITAE
: Full Name: Zhimin Wang
: Address: Allegheny-Singer Research Institute, West Penn Allegheny, Health
: System, South Tower, 320 East North Avenue, Pittsburgh, PA 15212-4772
| i********i
发帖数: 329 | 4 这次这个已经refer掉了，不好意思。能否发一封CV在我邮箱（包括email address），
这样我再有机会会主动发邮件给你。
谢谢了。
【在 w*****t 的大作中提到】
: 您好，不知道我的背景能否审到这片文章。我曾经审过一些小杂志，希望能够对我争取
: 这次机会有所帮助。详细的CV和研究背景如下。非常感谢您的考虑。我的工作邮箱：
: z****[email protected]
: 好像CV在这里粘贴很难看，不好意思。前一段时我的CV，后一段我的较详细的研究经历
: 。如有需要我更详细的背景情况，请跟我发信。
: 1
: CURRICULUM VITAE
: Full Name: Zhimin Wang
: Address: Allegheny-Singer Research Institute, West Penn Allegheny, Health
: System, South Tower, 320 East North Avenue, Pittsburgh, PA 15212-4772
| t*********1
发帖数: 2852 | |
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