Biology版 - Re: MCBJC, cell division (discussion?) |
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| ● Re: MCBJC, cell division, Jan 20 2003, by Assasin | ● Re: MCBJC, cell division (more discussion, 我又来乐) | | ● 求教：用total mRNA 做in vitro translation然后Western检测 | ● paper help/ call for MCBJC discussion | | ● in vitro transcription for short template | ● Re: MCBJC-call for discussion | | ● 请教一个拉丁用法 | ● Re: MCBJC, cellular neurobiology, Mar. 10, 2 | | ● 【请问】有commercially available的purified Cas9 Protein 吗？ | ● Call for MCBJC pathway, Mar. 31 | | ● Cell free expression system表达膜蛋白质好用不好用 | ● Re: Apoptosis---Re: Call for MCBJC dicus | | ● 如何quantify in vitro transcription产量呢？ | ● MCBJC(Neurogenesis/adult neural stem cell) | | ● MCBJC, cell division, discussion | ● MCBJC(Presenilin cofactors in r-secretase) |
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M****e
发帖数: 70 | 1 come on, show time, folks. so should we all email assassin back and corrupt
his mail box? i won't discuss all of the questions listed by assasin. actually,
to read papers on Cell is always headache, particularly manuscripts by
biochemistry/cell biology people.
Fig2A shows that purified separase can utilize in vitro translated(IVT) hSCC1
as well as endogenous hSCC1 as substrate in an in vitro cleavage assay. I think
the IVT sample (in RRL?) should be purified first (avoid incomplete translation), |
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| 相关主题 |
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| ● MCBJC(Presenilin cofactors in r-secretase) | ● 【请问】有commercially available的purified Cas9 Protein 吗？ | | ● MCBJC paper for discussion-042803 | ● Cell free expression system表达膜蛋白质好用不好用 | | ● Re: GST-Fusion 蛋白提纯求助!!! | ● 如何quantify in vitro transcription产量呢？ | | ● Re: 略感失望 | ● MCBJC, cell division, discussion | | ● Re: MCBJC, cell division, Jan 20 2003, by Assasin | ● Re: MCBJC, cell division (more discussion, 我又来乐) | | ● 求教：用total mRNA 做in vitro translation然后Western检测 | ● paper help/ call for MCBJC discussion | | ● in vitro transcription for short template | ● Re: MCBJC-call for discussion | | ● 请教一个拉丁用法 | ● Re: MCBJC, cellular neurobiology, Mar. 10, 2 |
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