L****S
发帖数: 76 | 1 Dear all,
I got another problem for Image J. My boos asked me to score TUNEL staining
for apoptosis, that means I need to score almost 1000 DAPI (nucleus) to see
among them how many TUNEL positive cell according to her. That is a lot of
scoring under microscope.
I wonder whether I can take images and use Image J to score DAPI (I just
need to score TUNEL).
Is there anyone knows how to use Image J to score DAPI?
Thank you.
Lei |
o**i
发帖数: 1165 | 2 难道google不到imager J的说明么？
staining
see
【在 L****S 的大作中提到】
: Dear all,
: I got another problem for Image J. My boos asked me to score TUNEL staining
: for apoptosis, that means I need to score almost 1000 DAPI (nucleus) to see
: among them how many TUNEL positive cell according to her. That is a lot of
: scoring under microscope.
: I wonder whether I can take images and use Image J to score DAPI (I just
: need to score TUNEL).
: Is there anyone knows how to use Image J to score DAPI?
: Thank you.
: Lei
|
b*****l
发帖数: 9499 | 3 赞 boos :)
建议先用 DAPI image 来做 thresholding 找出每个 nuclei，可能还需要做
segmentation 来区分开相邻的 nuclei，这个用 watershed 比较好。然后用 nuclei
的结果做 mask 来处理 TUNEL 的图像（如果背景干扰比较大，而且背景跟信号有叠加
，可以先去一遍背景），就可以得出每个 nucleus 里的 TUNEL 信号来。把这 1000+
nuclei 的 TUNEL 信号统计起来画成一个分布，然后就可以用分峰软件准确地找到两个
峰，算出面积，就可以很准确地得出 apoptosis rate。同时，也可以找到最佳的
threshold 点来分 TUNEL 信号，这样就可以把原始图上的每个细胞核都标为 pos 或者
neg。这样做的好处是根据分峰结果可以算出两类错误分别是多少。
这个流程的每一步，都是很规范很常用的工具，可以 google 到 ImageJ 的代码。不知
道有没有人集成过，反正我到最后还是懒得集成了。
staining
see
【在 L****S 的大作中提到】
: Dear all,
: I got another problem for Image J. My boos asked me to score TUNEL staining
: for apoptosis, that means I need to score almost 1000 DAPI (nucleus) to see
: among them how many TUNEL positive cell according to her. That is a lot of
: scoring under microscope.
: I wonder whether I can take images and use Image J to score DAPI (I just
: need to score TUNEL).
: Is there anyone knows how to use Image J to score DAPI?
: Thank you.
: Lei
|
L****S
发帖数: 76 | 4 Thank bigsail (河马·轩辕三光) very much. I will try your method. |
